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Here’s the complete code:

Sub runMacro()
Application.Wait “16:54:00”
addValues
End Sub

Sub addValues()
Dim lastrow As Long, i As Long, sum As Long
lastrow = Sheets(“Data”).Cells(Rows.Count, 1).End(xlUp).Row
sum = 0
For i = 2 To lastrow
sum = sum + Cells(i, 2)
Next i
MsgBox “The total is ” & sum
End Sub

Private Sub Workbook_Open()
Application.Wait “16:54:00”
addValues
End Sub

Further Reading:

Application.Wait Method in Excel